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Objective:To explore the mediating role of mindfulness in part-time nursing faculty between intrinsic motivation and work engagement.Methods:A total of 376 part-time nursing faculty were investigated with Work Preference Inventory, Five Facet Mindfulness Questionnaire-Short Form and Utrecht Work Engagement Scale. SPSS 25.0 statistical software was used to perform t test on the data. Results:The intrinsic motivation of part-time nursing faculty positively predicted work engagement ( β=0.74, P<0.001). The positive predictive effect of intrinsic motivation on job engagement was weaker than before ( β=0.55, P<0.001). Mindfulness as an intermediary variable reduced the positive predictive effect of intrinsic motivation on work engagement from 0.979 to 0.504. The indirect effect of intrinsic motivation on work engagement through mindfulness was significant (path coefficient=0.50, P<0.001), and the intermediary effect accounted for 48.51% of the total effect. Conclusion:Mindfulness plays an intermediary role between intrinsic motivation and work engagement of part-time nursing faculty. Paying attention to the mindfulness level of part-time nursing faculty plays a positive role in improving their work engagement level.
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<p><b>OBJECTIVE</b>To evaluate the feasibility of detecting macrophage content on atherosclerotic plaques by optical coherence tomography (OCT) technique.</p><p><b>METHODS</b>Thirty New Zealand white rabbits were equally divided into 3 groups at random: Control group (fed normal rabbit chow, n = 10); lipid diet group (fed regular chow supplemented with cholesterol, n = 10) and balloon injury+ lipid diet group (balloon catheter injury of the common carotid artery after 2 weeks lipid diet, n = 10). After 12 weeks, all rabbits underwent pharmacological triggering with Chinese Russell's viper venom (CRVV, 15 mg/kg, i.p.) and histamine (0.02 mg/kg, i.v.). Common carotid arteries were detected with OCT and the Movat pentachrome stain respectively. OCT and histological examination results were compared and the correlation was analyzed.</p><p><b>RESULTS</b>The intra thickness measured by Movat pentachrome stain and by the OCT was (15.2 ± 0.9) µm and (20.2 ± 7.6) µm, the medial thickness was (434.2 ± 86.5) µm and (453.8 ± 87.2) µm, the plaque thickness was (330.2 ± 87.1) µm and (392.2 ± 134.5) µm, the fibrous cap thickness was (58.3 ± 5.6) µm and (61.2 ± 4.9) µm, respectively (all P > 0.05). The normalized standard deviation of the OCT signal (NSD) was compared with immunohistochemical detection. The OCT signal within the cap is relatively homogeneous for low macrophage density in high lipid diet group. For the raw OCT data, a correlation of r = 0.846 (P < 0.01) was found between OCT NSD and a CD68 area<10%, whereas for the base 10 logarithm OCT data, a correlation of r = 0.646 (P < 0.05) was found between OCT NSD and a CD68 area<10%. In balloon injury + high lipid diet group, the OCT signal within the cap was relatively heterogeneous for high macrophage content. For the raw OCT data, a correlation of r = 0.906 (P < 0.01) was found between OCT NSD and a CD68 area >10%, whereas for the base 10 logarithm OCT data, a correlation of r = 0.593 (P < 0.05) was found between OCT NSD and a CD68 area >10%. For the raw OCT signal NSD, a range of NSDs (7.12% to 7.35%) demonstrated 100% sensitivity and specificity (Kappa value 1.0) for differentiating caps containing >10% CD68 staining. For the base 10 logarithm OCT signal, NSD values ranging from 7.81% to 7.92% provided 70% sensitivity and 75% specificity (value 0.44) for identifying caps containing >10% CD68 staining.</p><p><b>CONCLUSIONS</b>OCT is an effective tool to determine macrophage content in this model. OCT imaging can clearly visualize different types of atherosclerotic plaques and provide detailed information on plaque characteristics.</p>
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Animals , Rabbits , Carotid Artery, Common , Cholesterol , Fibrosis , Macrophages , Plaque, Atherosclerotic , Sensitivity and Specificity , Tomography, Optical CoherenceABSTRACT
Objeetive To investigate protective effect of trimetazidine on myocardial ischemia/reperfusion injury (MIRI) in rats.Methods Forty Wistar rats were randomly divided into MIRI group (n =10 rats),trimetazidine high-dosage group (n =10 rats; 20 mg/kg),trimetazidine low-dosage group (n=10 rats; 10 mg/kg),and the normal control group (n =10 rats).After MIRI,hemodynamic changes were observed,the concentration of IL-6 and TNF-α was determined,and the cardiac muscle histology under the microscope was observed.Results Hemodynamic studies:Compared to the indices LVSP(198 ±35.5) mmHg,LVEDP (17 ±9.18) mmHg,+ dp/dt max (11050 ± 1517.4) mmHg/s,and-dp/dtmax (9175± 1900) mmHg/s] in the sham-operated group,the indices [LVSP (143 ± 24.5) mmHg,LVEDP (37.5 ±7.16)mmHg,+ dp/dtmax (7450 ± 1755.1) mmHg/s,and-dp/dtmax (6075 ± 1641) Hg/S] in the MIRI group,the indices [LVSP (154.5 ± 31.1) mmHg,LVEDP (31.3 ± 12.6) mmHg,± dp/dtmax (8527.7 ±2251.5) mmHg/s,and-dp/dtmax (6694 ± 2242.2) mmHg/s] in the trimetazidine low-dosage (10 mg/kg)group,the indices[LVSP (168.3 ± 17.6) mmHg,LVEDP (28 ± 10.05) mmHg,+ dp/dtmax (9213.6 ±1747) mmHg/s,and-dp/dtmax (7568 ± 1462.4) mmHg/s] in the trimetazidine high-dosage (20 mg/kg)group,left ventricular remodeling end diastolic pressure (LVEDP),left ventricular systolic pressure (LVSP),and left ventricular pressure maxial rate of rise and fall (± dp/dtmax) were significantly decreased.Compared to the MIRI group,LVSP and ± dp/dtmax in the trimetazidine high-dosage (20 mg/kg)group were significantly increased (P ＜ 0.01),and myocardial damage of MIRI group was more severe in microscope.Compared to the sham-operated group [IL-6 (2556.5 ± 662.9) ng/ml,and TNF-α (134 ± 73.7)ng/ml],the corresponding indices [IL-6 (3664.0 ± 995.7) ng/ml,and TNF-α (443 ± 22.1) ng/ml] in the MIRI group,[IL-6 (3692.8 1545.2) ng/ml,and TNF-α (295 ± 24.2) ng/ml] in the trimetazidiue low-dosage (10 mg/kg) group,and[IL-6(2654.8 ±681.7) ng/ml,and TNF-α(230 ±7.8) ng/ml]in the trimetazidine high-dosage (20 mg/kg) group,the levels of IL-6 and TNF-α were significantly increased.Compared to the MIRI group,the levels of IL-6 and TNF-α were significantly decreased in the trimetazidine high-dosage (20 mg/kg) group (P ＜ 0.01).Conclusions The high-dosage (20 mg/kg) of trimetazidine had a protective effect on myocardial ischemia-reperfusion injury.
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Objective To establish an animal model of vulnerable plaque in abdominal aorta by pharmacological triggering of atherosclerosis in rabbits. Methods Thirty New Zealand white rabbits were randomly and equally divided into 3 groups: group A, receiving balloon injury together with high lipid diet; group B, receiving high lipid diet only, and group C, receiving regular diet only. After feeding for 12 weeks, all the rabbits in three groups underwent pharmacological triggering with Chinese Russell's viper venom (CRVV) and histamine to induce the rupture of the plaque and the formation of thrombosis. Results In 8 survival rabbits of group A, ten lesions with the stenotic degree over 50% were found, which had large lipid core with the thickness of fibrous cap being thinner than 65μm, by which these ten lesions were judged as vulnerable plaques. In 9 rabbits of group B, only 3 vulnerable plaques could be determined, while no single vulnerable plaque could be identified in the rabbits of group C. Conclusion For establishing an animal model of vulnerable plaque in abdominal aorta in rabbits, balloon injury combined with high lipid diet is a feasible and reliable method.
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Objective To establish an animal model of vulnerable plaque in abdominal aorta by pharmacological triggering of atherosclerosis in rabbits.Methods Thirty New Zealand white rabbits were randomly and equally divided into 3 groups: group A,receiving balloon injury together with high lipid diet;group B,receiving high lipid diet only,and group C,receiving regular diet only.After feeding for 12 weeks,all the rabbits in three groups underwent pharmacological triggering with Chinese Russell’s viper venom(CRVV) and histamine to induce the rupture of the plaque and the formation of thrombosis.Results In 8 survival rabbits of group A,ten lesions with the stenotic degree over 50% were found,which had large lipid core with the thickness of fibrous cap being thinner than 65?m,by which these ten lesions were judged as vulnerable plaques.In 9 rabbits of group B,only 3 vulnerable plaques could be determined,while no single vulnerable plaque could be identified in the rabbits of group C.Conclusion For establishing an animal model of vulnerable plaque in abdominal aorta in rabbits,balloon injury combined with high lipid diet is a feasible and reliable method.